Comparative Biological Pathotyping of Newcastle Disease Virus Sub-genotypes VIIg, VIIh, and VIIi in Specific Pathogen Free (SPF) Chickens

Newcastle Disease (ND) is an infectious viral disease in poultry caused by the virus (NDV). The NDV that causes ND cases Indonesia genotype VII with various sub-genotypes. This study was conducted to compare vivo pathogenicity of sub-genotypes VIIg, VIIh, and VIIi specific pathogen free (SPF) chickens. isolates were isolated identified as VIIi. level known calculating mean death time (MDT) intravenous index (IVPI). comparations lesions analyzed descriptively statistically based on clinical signs, ratio lymphoid organ weight, hematoxylin-eosin (HE). Isolate confirmation harvesting lung organs. detected using Reverse Transcription-Polymerase Chain Reaction (RT-PCR) primers fusion (F) fragment gene. sequence compared other NDVs from GenBank Analyzed MEGA-6 software. analysis results showed VIIh virulent strains due MDT <60 hours IVPI value 3.0, organs showing atrophy lymphocyte depletion. On hand, VIIg sub-genotype a less strain because it had 0.0 >60 hours, mild Based at cleavage site Sub-genotypes (112RRQRRF117) GVIIi (112RRQKRF117) strains, but Sub-genotype (112GRQGRL117) virulent.